| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Feuzeu Mekoue, Laurielle Kel (13)
|
MASSC |
54.60 |
55.86 |
1.26 |
| 2 |
Brandt, Mackenzie A (13)
|
MAUNSS |
54.69 |
56.89 |
2.20 |
| 3 |
Shema, Erin O (15)
|
MAUNSS |
55.02 |
57.00 |
1.98 |
| 4 |
Aguirre, Madeleine T (17)
|
MASSC |
55.95 |
58.80 |
2.85 |
| 5 |
Jones, Elizabeth G (15)
|
MASSC |
1:00.76 |
1:00.11 |
-0.65 |
| 6 |
Cappola, Christina M (14)
|
MASSC |
1:03.19 |
1:01.62 |
-1.57 |
| 7 |
Jones, Laura G (15)
|
MASSC |
59.00 |
1:01.75 |
2.75 |
| 7 |
Slupianek, Helena (14)
|
MASSC |
1:02.64 |
1:01.75 |
-0.89 |
| 9 |
Wilson, Mekhi S (13)
|
MASSC |
57.64 |
1:02.45 |
4.81 |
| 10 |
Paglia, Sophia B (13)
|
MASSC |
1:07.00 |
1:04.95 |
-2.05 |
| 11 |
Scott, Ella G (13)
|
MASSC |
1:04.36 |
1:06.07 |
1.71 |
| 12 |
Mitchell, Julia G (14)
|
MASSC |
1:08.17 |
1:11.04 |
2.87 |
|
Hipp, Alexa R (16)
|
MASSC |
57.75 |
NS |
- |